Optimization of Media Composition for Maximum Growth of Probiotic Lactobacillus fermentum NBC-08 Using Response Surface Methodology

Abstract

In this study, it was aimed to determine the medium where Lactobacillus fermentum microorganisms with probiotic properties grow most efficiently by using Response Surface Method (RSM). Studies have been conducted on a 500 ml erlenmeyer scale, the medium optimization of the Lactobacillus fermentum strain was carried out according to the Central Composite Design (CCD) included in RSM. The effects of glucose, yeast extract, inorganic salts, and Tween 80 were investigated on the growth rate of the L. fermentum NBC-08 strain. Samples were taken at regular intervals from the erlenmeyer flask and the number of viable cells was measured by planting them in petri agar medium. In the study, the number of viable cells log10 (cfu/ml) was chosen as the response variable. As a result of the study, it was concluded that glucose and yeast extract are absolutely essential components in the medium. The optimum medium composition was found as 96.06 g/L glucose, 40.76 g L-1 yeast extract, 19.43 g L-1 inorganic salts, and 11.01 ml/L Tween 80. The production of the maximum L. fermentum strain was determined as 10.75 log10 (cfu ml-1). It is predicted that this study will make positive contributions to the fermentation conditions and medium optimization studies for production of lactic acid bacteria