Abstract
Biocemented Martian regolith simulant (MRS) columns were developed using a microalga, Thraustochytrium striatum. Response surface methodology was used to maximize the unconfined compressive strength (UCS) of biocemented MRS columns towards regolith particle size, cell biomass loading, and nutrient (CaCl2/urea) loading. Overall, the UCS increased with the increase of cell biomass loading while the MRS of medium particle size achieved the highest UCS. But the nutrient loading didn’t show a consistent effect. The maximum UCS reached 603.83 kPa with 0.25 ∼ 0.425 mm particle size, 3.8 g/100 mL cell biomass loading, 5.83 g/100 mL CaCl2 loading, and 14.18 g/100 mL urea loading.
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