Optimization of lipase extraction from pequi seed ( Caryocar brasiliense Camb.)

Abstract

The optimization of extraction conditions (time and volume) of lipase from pequi seed using ammonium sulfate and additives (tween 80 and calcium chloride) was studied. The results of statistical analysis showed significant effect of time and volume parameters on response variables enzymatic activity and protein recovery, where the highest activity of 1726.18 U was obtained in 3.0 ml of extraction solution for 25 min and 98.74% recovery in 2.5 ml for 15 min. The response surface indicated a better performance for both variables as time increased and volume decreased. The coefficients of determination and correlation graph validated the planning data. Thus, the optimal values of time and volume to obtain the greatest enzymatic activity in the lipase extraction process from pequi seed were fixed in 25 min and 3.0 ml of extraction solution composed of ammonium sulfate + tween 80. Practical applications An alternative plant lipase source obtained from pequi seed has been investigated for the first time. The results show that experimental conditions established in this work allow the efficient extraction of lipase, an enzyme with potential for application in several industrial segments.