Optimization of key factors affecting bio-hydrogen production from sweet potato starch

Abstract

Key factors affecting the bio-hydrogen production from sweet potato starch were optimized by response surface methodology (RSM) with Box-Behnken design. Sweet potato concentration, FeSO4 concentration, and initial pH were identified by Plackett-Burman design as the significant factors. The path of steepest ascent and Box-Behnken design were subsequently applied to optimize the levels of the significant variables. The results indicated that the concentration of sweet potato starch, and the interaction between pH and FeSO4 concentration significantly affected the cumulative hydrogen production. The maximum cumulative hydrogen production of 3501 mL/L was achieved under the optimum conditions of the initial pH of 6.05, sweet potato starch concentration of 27.63 g/L, and FeSO4 concentration of 63.17 mg/L.