Abstract
Soil samples were collected from the feather dumped area where Bacillus pumilus was isolated and used for keratinase production and keratinolytic activity. In the optimization study, optimal condition for enzyme production was observed at 144 h, pH 7, temperature 37°C. The organism was utilized for feather degradation study. The maximum degradation of 57% was obtained at 37°C, pH 7 and 6 days incubation. The size of keratinase was determined by SDS- PAGE and was observed as 52 KDa.
Highlights
As a by-product of poultry waste, million tons of feathers are produced annually from the poultry Industries[1]
Soil samples were collected from the feather dumped area where Bacillus pumilus was isolated and used for keratinase production and keratinolytic activity
The present study was conducted to use Bacillus pumilus isolated from the feather contaminated soil, it was optimized for production of keratinase enzyme and the organism was used for effective degradation of feather
Summary
As a by-product of poultry waste, million tons of feathers are produced annually from the poultry Industries[1]. Feather pollution can be controlled by alternate use of feather wastes[5]. Many researchers isolated microorganisms like fungi, actinomycetes, and several bacterial species such as Bacillus sp., Pseudomonas sp., which are able to produce keratinase which indicates that they are capable of utilising the keratin found in the feather with a metabolic reaction[8,9,10,11]. Microbial degradation of feather improves the nutritional quality of the feather meal and it is a good alternative for Physio chemical treatment[12,13]. The present study was conducted to use Bacillus pumilus isolated from the feather contaminated soil, it was optimized for production of keratinase enzyme and the organism was used for effective degradation of feather
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