Abstract
Abstract The development of a process that could recover biofuel from industrial cellulose waste can not only reduce the negative environmental impacts by using fossil fuels, but also bring a green idea for the waste's disposing. In this study, hydrothermal pretreatment was optimized for cassava anaerobic residue, a cellulosic waste from cassava ethanol industry, to co-utilize xylose and glucose for producing bioethanol. The effect of the main pretreatment conditions, namely, temperature, solid content and time, was explored for the highest recovery of xylose in prehydrolysate and glucose in enzymatic hydrolysate. The single factor experiment results showed that the conditions for maximum xylose recovery in prehydrolysate and glucose recovery in enzymatic hydrolysate were 60 °C, 75 min, 10% solids and 160 °C, 75 min, 10% solids, respectively. Whereafter, response surface methodology (RSM) was applied to further optimize the pretreatment conditions for the maximum theoretical ethanol production through utilizing both xylose and glucose. A treatment at 163 °C, for 59 min and with 9.5% solids was found optimal, with the highest ethanol production of 20.2 mg·g−1 raw material. Furthermore, in order to assess the impacts of the pretreatment on cassava anaerobic residue, the changes in crystallinity and morphology for untreated and pretreated solids were investigated.
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