Abstract
Hydrogen production from sucrose in a granule-based upflow anaerobic sludge blanket (UASB) reactor was optimized through employing response surface methodology (RSM) with a central composite design in this study. The individual and interactive effects of influent sucrose concentration ( S in) and hydraulic retention time (HRT) on anaerobic hydrogen production were elucidated. Experimental results show that a maximum hydrogen yield of 1.62 mol-H 2/mol-hexose was obtained under the optimum conditions of S in 14.5 g/L and an HRT 16.4 h. The hydrogen yield was individually dependent on S in and HRT, while their interactive effect on the hydrogen yield was not significant. Throughout the experiments the hydrogen content fluctuated between 25.9% and 50.0%, but free of methane. Ethanol, acetate and butyrate were the main aqueous products and their yields all correlated well with S in and HRT, indicating a mixed-type fermentation in this UASB reactor.
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