Abstract
In order to ascertain the optimal hormone combination for Chinese fir (Cunninghamia lanceolata) leaf cuttings, an L16(4)4 orthogonal test of 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) (0, 10, 30, or 50 mg · L−1 of each exogenous hormone) immersion for 5, 10, 15, or 20 min was conducted. Callus initiation rate and rooting promotion rate were mainly affected by treatment time, root length increase by 6-BA concentration, and bud germination rate and plantlet formation rate by NAA concentration. The expected optimal combination for callus initiation rate was 50 mg · L−1 6-BA + 0 mg · L−1 NAA + 30 mg · L−1 IBA + 10 min; for rooting promotion rate, it was 0–10 mg · L−1 6-BA + 10 mg · L−1 NAA + 30 mg · L−1 IBA + 20 min; for bud germination rate, it was 50 mg · L−1 6-BA + 0–10 mg · L−1 NAA + 0–30 mg · L−1 IBA + 20 min; and for seedling formation rate for No. 4, it was 10 mg · L−1 6-BA + 10 mg · L−1 NAA + 0 mg · L−1 IBA + 20 min. Light microscopy image analysis revealed that a cluster of primordial cells was produced 40 days after cutting, and mastoid cells developed into peninsula cells in calli that were cultured for 50 days.
Highlights
5, 10, 15, or 20 min was conducted
Studies have been conducted on the establishment of an efficient tissue culture system for rapidly propagating Chinese fir; these have focused on callus induction and axillary bud and/or shoot differentiation and regeneration[12,13,14]
The range analysis indicated that indole-3-butyric acid (IBA) and 6-BA were the determinants of callus initiation rate and root growth, respectively
Summary
5, 10, 15, or 20 min was conducted. Callus initiation rate and rooting promotion rate were mainly affected by treatment time, root length increase by 6-BA concentration, and bud germination rate and plantlet formation rate by NAA concentration. Studies have been conducted on the establishment of an efficient tissue culture system for rapidly propagating Chinese fir; these have focused on callus induction and axillary bud and/or shoot differentiation and regeneration[12,13,14]. Pang et al.[13] investigated the effects of culture and the indole-3-butyric acid (IBA)/NAA combination ratio on root induction in tissue-cultured seedlings, and selected the optimal combination. Most of these tissue-cultured materials were explanted from stem segments, and the propagation requirements of branch cuttings from superior mother clones are difficult to meet. Tissue culture is expensive, requires the management of professional workers, and induction culture related to calli is difficult; they have been subcultured for no more than two or three generations[7, 15]
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