Abstract
This study was conducted to enhance the yield performance of Calocybe indicathrough optimization of the cultivation parameters by utilizing cheaper substrates that are available in Tamil Nadu, India. The total fresh mushroom yield obtained with a change in spawn substrate, spawn running bed substrate (SRBS), sterilization procedure and casing soil with different amendments were studied. Sorghum grainsprovided the highest yield of 1.58 ± 0.06 g fresh mycelia after 17 ± 0.75 days of incubation. The protein, fat, carbohydrate, dietary fiber, moisture and ash content of mushrooms were also analyzed. The maximum amount of nutritive value was obtained from the paddy straw and the lowest from sugarcane leaves as substrate. The fat, protein, carbohydrate, dietary fiber, moisture and ash (g/100 g dried matter) values of C. indica were 0.66 ± 0.02 g, 31.29 ± 1.56 g, 58.40 ± 1.75 g, 38.21 ± 1.91 g, 84.91% and 8.47 ± 0.25 g, respectively, when cultivated in paddy straw. Furthermore, the chemical sterilization yielded 1.16 kg of mushroom with a contamination rate of <8 of 30 bags. The different chemical compositions were used in the preparation of casing soil; however, it produced the highest yield of 1648 ± 49.44 g/kg (w/w) for paddy straw. Key words: Calocybe indica, chemical sterilization, growth parameters, physical sterilization, substrate.
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