Abstract
Polyhydroxyalkanoates (PHAs) are intracellularly synthesized by bacteria as carbonosomes that exhibit biodegradable thermoplastics and elastomeric properties. The use of cheaper edible oils as a source of carbon assists in the reduction of the production cost of such biopolyesters. In this work, different edible oils, such as groundnut oil (GNO), mustard oil, sesame oil, and soybean oil (SBO) were used to check their effect on PHA production from Pseudomonas aeruginosa EO1 (MK049902). Pseudomonas aeruginosa EO1 was used in a two-stage production system. In the first stage, bacterial growth was favored and, in the second, PHA was synthesized. GNO was found as the best carbon source for PHA production. The use of 2% (v/v) GNO, rich in saturated fatty acids, allowed PHA content of 58.41% and dry cell weight (DCW) of 10.5g/L at pH7 and temperature 35°C for 72h. Groundnut has a high potential for oil production and for the diversification of co-products with some potential of value aggregation. Such a perennial and sustainable species will almost certainly meet the criteria for becoming a significant commercial oilseed crop. Fourier transform infrared spectroscopy (FTIR) spectra showed strong characteristic bands at 1,282, 1,725, 2,935, 2,999, and 3,137cm−1 for the PHA polymer. Gas chromatography-mass spectrometry (GC-MS) detects the presence of PHA copolymers.
Highlights
Products that arise from synthetic plastic generate serious difficulties in solid waste disposal when dumped into the environment
Bacterial isolate P. aeruginosa EO1 was investigated for its taxonomic position
The closest Pseudomonads strains showed about 98–100% of shared sequence identity with EO1
Summary
Products that arise from synthetic plastic generate serious difficulties in solid waste disposal when dumped into the environment. PHA are good members of biodegradable polyesters for the production of eco-friendly, biodegradable plastics It is synthesized inside a diversity of microorganisms as a carbon and energy reserve, and its synthesis depends on environmental stress conditions e.g., a deficiency of nitrogen, phosphate, or oxygen (Lee, 1996a). The second group includes the bacteria that do not need nutritional limitations for PHA synthesis during bacterial cell growth, for instance, Alcaligenes latus, A. vinelandii, Pseudomonas putida, c47T2, and r-E. coli (Lee, 1996a). Plant oils, such as soybean, palm, and corn oil are suitable carbon substrates for PHAs synthesis because of their low cost compared to most sugars (Ciesielski et al, 2015). We report: (1) The taxonomic classification of the P. aeruginosa strain EO1 (MK049902); (2) The PHA accumulation ability of P. aeruginosa strain EO1 utilizing edible oils; (3) and describe the influence of nutritional conditions on its growth; and (4) characterization of produced PHA
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