Optimization of Fibrinolytic Enzyme from Streptomyces althioticus BN22

Abstract

Fibrinolytic enzyme plays an important place in the medical field for cardiovascular disease. Only few studies have reported the fibrinolytic enzyme production from marine actinomycetes, and Streptomyces sp. In this study 114 actinomycetes isolates from different marine soil samples were isolated by using starch casein agar media. These 114 actinomycetes strains were tested for fibrinolytic activity by fibrin plate method. 14 isolates showed fibrinolytic activity amongst the 114 actinomycetes strains, and this was identified based on the clear zone of formation around the colonies. The potent strain of Streptomyces althioticus BN22 was isolated and this was confirmed by 16SrRNA sequencing. The optimization of nutritional and physical parameters for fibrinolytic enzyme production from marine soil was studied for the isolate Streptomyces althioticus. The effects of nutritional and physical parameters were studied for the optimum production of a fibrinolytic enzyme from Streptomyces althioticus BN22 by submerged fermentation. The effects of initial pH, temperature and incubation time were studied. The maximum enzymatic activity was obtained with an initial pH7.5 (45,765 Eu/ml), incubation temperature 50°C (50,827 Eu/ml) and incubation time for (41,175 Eu/ml). The effects of various carbon and nitrogen sources were studied. The results indicated that glucose (51,570 Eu/ml) as carbon source and yeast extract (54,625 Eu/ml) as nitrogen source showed the highest fibrinolytic enzyme activity among them. Thus this study showed its originality by focus, that marine Streptomyces althioticus is an important source for fibrinolytic enzyme production.