Optimization of fermentation media and growth conditions for microbial xylanase production.

Bushra Kalim,Nazish Mazhar Ali

doi:10.1007/s13205-016-0445-3

Bushra Kalim, Nazish Mazhar Ali

Open Access

https://doi.org/10.1007/s13205-016-0445-3

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Journal: 3 Biotech	Publication Date: Jun 3, 2016
Citations: 18	License type: CC BY 4.0

Affiliation: Government College University, Lahore

Abstract

Efficiency of cellulase-free xylanases is one of the determining factors in paper and pulp industries. Use of microbes which can produce cellulase-free xylanases may help to overcome the current challenges in kraft pulp processing. Isolation and screening of microorganisms from local samples offers a possibility for obtaining the potential microbes for this purpose. This research was therefore aimed to collect, screen, characterize and identify potential cellulase-free xylanase producers. A total of 313 microbial isolates were collected while using selective media (EBAM and XAM) to determine the xylanolytic potential of microbes. Qualitative and quantitative analyses were performed and finally 11 bacterial and 6 fungal strains were selected for characterization and identification. The potential isolates were identified as Bacillus pumilus (388.82 U/mg), Bacillus safensis (385.26 U/mg), Aspergillus flavus (493.33 U/mg) and Aspergillus niger (419.33 U/mg). Optimization of the microbial strains while using agro-industrial waste is suggested.

Highlights

Xylanases are of great importance in a number of industrial processes and the increasing trend towards an environment friendly industrialization has paved its ways
The potential isolates were identified as Bacillus pumilus (388.82 U/mg), Bacillus safensis (385.26 U/mg), Aspergillus flavus (493.33 U/mg) and Aspergillus niger (419.33 U/mg)
Out of the eleven bacterial and six fungal isolates that have been characterized for their cellulase free xylanase activity, Bacillus pumilus (BS131) and Bacillus safensis (BS37) are potential bacterial isolates due to their significant xylanase and minimum cellulase production

Summary

Introduction

Xylanases are of great importance in a number of industrial processes and the increasing trend towards an environment friendly industrialization has paved its ways. Xylans are heterogeneous compounds and require synergistic reaction of hydrolytic enzymes for their complete degradation. Among these enzymes, endo-b-1,4-xylanases (EC.3.2.1.8) are the most important xylanolytic enzymes which cleave off the internal glycosidic bonds in xylan backbone, reducing the degree of polymerization (Biely 1985; Polizeli et al 2005). To contribute in the improvement of biodegradation of hemicelluloses through microbial co-cultures, exploration, selection and characterization of potential cellulase-free xylanase producing microbes may have important roles. The production of cellulase-free xylanase is of crucial importance in paper and pulp industry. The objective of the present study was to collect, screen, identify and characterize cellulase-free xylanase producing locally isolated microbial strains

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