Abstract
Glucose isomerase is an enzyme widely used in food industry for producing high-fructose corn syrup. Many microbes, including Bacillus megaterium, have been found to be able to produce glucose isomerase. However, the number of studies of glucose isomerase production from Bacillus megaterium is limited. In this study, we establish the optimal medium components and culture conditions for Bacillus megaterium glucose isomerase production by evaluating the combined influence of multiple factors and different parameters via Plackett–Burman design and response surface methodology in Modde 5.0 software. The optimized conditions, which were experimentally confirmed as follows: D-xylose (1.116%), K2HPO4 (0.2%), MgSO4·7H2O (0.1%), yeast extract (1.161%), peptone (1%), pH 7.0, inoculum size 20% (w/v), shaking 120 rpm at 36.528°C for 48 hours, give rise to production of highest activity of glucose isomerase (0.274 ± 0.003 U/mg biomass). These results provide additional important information for future development of large-scale glucose isomerase production by Bacillus megaterium.
Highlights
High-fructose corn syrup (HFCS) is a mixture of glucosefructose which is widely used as a low-calorie sweetener in food industry
Known as D-glucose isomerase and D-xylose isomerase, is the intracellular enzyme which catalyzes the isomerization of glucose to fructose and xylose to xylulose; it accelerates the sweetness of food product and plays an important role is HFCS production
We investigate the combined influence of multiple factor of cultivation parameters, such as nutrient composition and cultivation conditions, on production of glucose isomerase and optimize the main cultivation parameters for Bacillus megaterium to produce maximal glucose isomerase activity using the Plackett– Burman design and response surface methodology in Modde 5.0 software
Summary
High-fructose corn syrup (HFCS) is a mixture of glucosefructose which is widely used as a low-calorie sweetener in food industry. On the market, commercialized glucose isomerase products are sold as immobilized enzymes and cells Enzymatic activity of these products is regulated by a variety of factors such as the presence of metal cations, microbial sources, pH, and temperature. The combined influence of multiple factors of cultivation parameters, such as nutrient components (Dxylose, K2HPO4, MgSO4, peptone, and yeast extract) and cultivation conditions (rotation rate, pH, temperature, inoculum size, and cultivation time), on the biosynthesis of glucose isomerase in Bacillus megaterium has not been elucidated yet. We investigate the combined influence of multiple factor of cultivation parameters, such as nutrient composition and cultivation conditions, on production of glucose isomerase and optimize the main cultivation parameters for Bacillus megaterium to produce maximal glucose isomerase activity using the Plackett– Burman design and response surface methodology in Modde 5.0 software
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
