Abstract
In this paper, the optimization of the extraction/purification process of multiple components was performed by the entropy weight method (EWM) combined with Plackett–Burman design (PBD) and central composite design (CCD). We took the macroporous resin purification of Astragalus saponins as an example to discuss the practicability of this method. Firstly, the weight of each component was given by EWM and the sum of the product between the componential content and its weight was defined as the comprehensive score, which was taken as the evaluation index. Then, the single factor method was adopted for determining the value range of each factor. PBD was applied for screening the significant factors. Important variables were further optimized by CCD to determine the optimal process parameters. After the combination of EWM, PBD and CCD, the resulting optimal purification conditions were as follows: pH value of 6.0, the extraction solvent concentration of 0.15 g/mL, and the ethanol volume fraction of 75%. Under the optimal conditions, the practical comprehensive score of recoveries of saponins was close to the predicted value (n = 3). Therefore, the present study provided a convenient and efficient method for extraction and purification optimization technology of multiple components from natural products.
Highlights
Compared with the other resins in a comprehensive consideration of the recovery of each saponin, AB-8 macroporous resin possessed a great advantage for the seven saponins, so it was selected to optimize the purification process of total saponins
It has been reported that macroporous resin with weak polarity had a special selectivity for saponins, which was suitable for the purification of saponins or some organic substances from aqueous solution [20]
AB-8 macroporous resin had a strong selective enrichment effect on astragalosides, it had a significant advantage in the purification of astragalosides compared with other macroporous resins
Summary
Optimization of extraction and purification techniques of multi components in natural products has been extensively studied and developed [1,2]. When a method is applied for extracting or purifying the components of natural products, many factors need to be considered. The method of macroporous adsorption resin purification has been used for the enrichment of various natural ingredients, but there are numerous conditions such as elution flow rate, sample amount and concentrations of ethanol in elution reported in the literature when separating different components [3]. There are multiple factors in the extraction or purification methods, only a few have significant impacts on the final results. It is necessary to establish a suitable method to screen these significant factors
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