Optimization of Extraction of Bioactive Material-Polysaccharides from the Roots of <i>Taraxacum mongolicum</i> by Box-Behnken Experimental Design

Abstract

Dandelion (Taraxacum spp) is an medical active materials used in many traditional and modern herbal medical systems, its polysaccharides have many bioactivities. Taraxacum mongolicum is one of the most widely consumed as Chinese herbal medicine and root tea in Changbai Mountains area, Northeast China. In this study, a procedure for the extraction of polysaccharides from the root of T mongolicum(TMRPS) was established. Based on single-variable tests experiment to determine the ranges of the extraction parameters (extraction temperature (X1), extraction time (X2), and ratio of solvent to raw material (X3)), response surface methodology (RSM) was used to optimize for these extraction parameters (X1, X2, X3) enhancing the forward extraction efficiency of TMRPS by a three-level three-variable Box-Behnken experimental design. The results showed that the ranges of the extraction parameters were determined as 60°C-80°C(X1),1h-3h (X2),20-40(v/m)(X3) by single-variable tests and the maximal yield (9.01%) of TMRPS was reached at extraction temperature 70.2°C, extraction time 1.79 h and ratio of solvent to raw material 28.0 (v):1 (m). Convenient for experiment, appropriate readjustment of these extraction parameters was made. Under these readjusted conditions, the mean extraction yield of TMRPS was 8.85%, which was near to theoretical calculation and indicates that these results were agree closely with model predictions.