Optimization of extraction of antioxidant polysaccharide from Pleurotus ostreatus (Jacq.) P. Kumm and its cytotoxic activity against murine lymphoid cancer cell line.

Md Moyen Uddin Pk,Matiar Rahman,Mohammad Sayful Islam,Rabiul Islam Talukder,Rumana Pervin,Subhajit Dutta,David A Lightfoot

doi:10.1371/journal.pone.0209371

Abstract

The purpose of this study was to optimize the extraction method for polysaccharide from the fruiting bodies of Pleurotus ostreatus (Jacq.) P. Kumm and to assess the antioxidant and cytotoxic potentials of polysaccharide. In this investigation, polysaccharides from Pleurotus ostreatus (Jacq.) P. Kumm were extricated by utilizing the hot water. One-single factor and response surface methodology was established to optimize the extraction conditions for polysaccharide from Pleurotus ostreatus (Jacq.) P. Kumm. Examination of antioxidant activity of Pleurotus ostreatus polysaccharide (POP) was directed by utilizing 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2-azino-bis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) techniques. Cytotoxicity of POP was evaluated using an MTT assay. The experimental data were fitted to a quadratic equation utilizing multiple regression investigations, and the ideal conditions were as per the following: water/crude material proportion, 26.04 mL/g; an extraction time of 62.08 minutes; and an extraction temperature 70.5°C. Under such conditions, the polysaccharide yield was 5.32 ± 0.12% with the anticipated yield. POP showed good scavenging activity against DPPH radical (p<0.001, EC50 = 1036.38 μg/mL, R2 = 0.8313) and ABTS radicals (p<0.001, EC50 = 824.37 μg/mL, R2 = 0.8223), with a dose (p<0.001)-and-time (p<0.001) dependent cytotoxic potential on Ehrlich ascites carcinoma cell line in vitro. This demonstrated that polysaccharides (POP) had certain cancer prevention agent exercises. In this manner, these examinations give reference to additionally research and reasonable improvement of Pleurotus ostreatus (Jacq.) P. Kumm polysaccharide and POP may prove a useful therapeutic agent, due to its robust antioxidant and cytotoxic activity.

Highlights

P. ostreatus, the oyster mushroom, is a consumable mushroom, generally growing all over the world
The in vitro antioxidant activities of Pleurotus ostreatus polysaccharide (POP) were assessed on the base of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and 2,2-azino-bis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity
Impact of water to raw material ratio on the POP yield The polysaccharide was extracted at different water to raw materials ratios, with a fixed extraction time and extraction temperature at 60 ̊C and 70 min respectively

Summary

Introduction

P. ostreatus, the oyster mushroom, is a consumable mushroom, generally growing all over the world. Oyster mushrooms are a rich source of vitamins, amino acids, and minerals, as well as antioxidants[2]. In addition to their role in nutrition, they possess medicinal properties. Extraction proficiency of polysaccharide is influenced by several factors: extraction time, extraction temperature and water to material proportion. Their effects on the polysaccharide yield may be autonomous. Box-Behnken Design, a kind of RSM, was used to find optimum extraction parameters of polysaccharides extraction from Pleurotus ostreatus The objective of this investigation was to optimize the conditions for polysaccharide extraction from Pleurotus ostreatus (Jacq.) P. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used to measure in vitro role of cytotoxicity in Murine Lymphoid cancer cell line (EAC cell line)

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