Optimization of Extraction and Determination of Chloramphenicol in Livestock Meat Samples using Aqueous Two-Phase System ofn-propanol and Potassium Citrate Coupled with HPLC

Abstract

A slurry sampling flame atomic absorption spectrometric method for sequential determination of Cu and Fe in airborne particulate matter (APM) collected on glass fiber filters is proposed. The method optimization was carried out using a dry powdered sample (filter + APM) mass of 50 mg, dispersed in 0.5 mol L-1 nitric acid solution and under ultrasonic bath for 10 min. The limits of detection (n = 10) were 4 and 14 µg g-1 for Cu and Fe on the filter, respectively. The repeatability expressed as the relative standard deviation was better than 7 and 8% (n = 3) for Cu and Fe, respectively. The accuracy of the method was confirmed by analysis of the fly ash certified reference material (BCR 176R). The results obtained for six samples of APM using the proposed method showed no significant differences with those obtained after microwave-assisted acid extraction and determination by inductively coupled plasma mass spectrometry. The analytical method proved to be simple, fast and reliable.