Optimization of Extracellular Lipase Production in Colletotrichum Gloeosporioides by Solid State Fermentation

Abstract

Twenty two endophytic fungi isolated from oil seeds were screened for their ability to produce extracellular lipolytic enzymes in solid state fermentation. Among them, the most productive strain, identified as Colletotrichum gloeosporioides was cultivated for lipase production in solid state fermentation (SSF) using oil-mill residue as a solid support. The elaboration of extracellular lipases from C. gloeosporioides on different residual cheap oil substrates was tested. Among these, pongamia oil cake (POC) showed maximum lipase activity (983 U/g dry matter (DM)) followed by coconut oil cake (COC) (925 U/g DM). These two oil industrial residues were chosen for enhanced lipase production. The maximum enzyme yield was obtained when POC was impregnated with CDB (Czapek-Dox Broth) in the ratio of 1:1.5 and with (906 U/g DM). Triton X 100 as the extractant from the fermented solids yielded 1020 U/g DM. The optimum temperature for lipase secretion by C. gloeosporioides was found to be 25°C. Xylose as the carbon source and peptone as the nitrogen source were favorable for the secretion of the enzymes. Tween 60 served as a good lipid substrate for lipase production (1170U/g DM) when POC was the substrate. Amending the POC with Magnesium sulphate as a metallic ion source induced good lipase activity (1240 U/g DM) by C. gloeosporioides. The Sunflower oil was found to be best co-vegetable oil substrate to induce the enzyme (2560 U/g DM). Triton X100 served as the surfactant for lipase secretion in C. gloeosporioides. Thus, pongamia oil cake can be used as cheap substrate for enhanced extracellular lipase production by C. gloeosporioides .