Optimization of enzymatic process parameters for increased juice yield from carrot (Daucus carota L.) using response surface methodology

Abstract

The effects of enzyme concentration (50–650 mg/kg grated carrot), pectolytic and cellulolytic enzyme ratio (3:7–7:3), incubation time (30–150 min) and temperature (25–65 °C) on juice recovery and viscosity from grated carrot were studied. A central composite rotatable design (CCRD) was used in designing the treatment combinations of four variables at five levels. The process involved in treating the blanched grated carrot with mixture of crude pectolytic enzyme from Aspergillus foetidus and crude cellulolytic enzyme from Trichoderma ressi, keeping the samples at the desired time, followed by extraction of juice. Enzyme-treated grated carrot sample showed increased juice recovery as compared to control. A second-order response surface model adequately fitted the data. All the variables affected juice recovery and viscosity significantly. Enzyme concentration, pectolytic and cellulolytic enzyme ratio, incubation time and temperature had total and combined effect at linear, square and interactive level on both responses. The optimum condition was enzyme concentration, 210.7 mg/kg of grated carrot; pectolytic and cellulolytic enzyme ratio, 3.84:6.16; incubation time, 130 min and incubation temperature 47 °C. Under the optimal conditions, juice extracted from enzyme-treated grated carrot was 74.3% having juice viscosity 1.07 cP, corresponding to the increase in yield by 13.95% and decrease in viscosity by 0.45 cP.