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Abstract
Stranded driftwood feedstocks may represent, after pretreatment with steam explosion and enzymatic hydrolysis, a cheap C-source for producing biochemicals and biofuels using oleaginous yeasts. The hydrolysis was optimized using a response surface methodology (RSM). The solid loading (SL) and the dosage of enzyme cocktail (ED) were variated following a central composite design (CCD) aimed at optimizing the conversion of carbohydrates into lipids (YL) by the yeast Solicoccozyma terricola DBVPG 5870.A second-order polynomial equation was computed for describing the effect of ED and SL on YL.The best combination (ED = 3.10%; SL = 22.07%) for releasing the optimal concentration of carbohydrates which gave the highest predicted YL (27.32%) was then validated by a new hydrolysis. The resulting value of YL (25.26%) was close to the theoretical maximum value.Interestingly, fatty acid profile achieved under the optimized conditions was similar to that reported for palm oil.
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