Abstract
Abstract Cell wall disruption is an essential downstream processing step for improving the efficiency of lipid extraction from microalgae. Enzyme-assisted extraction of lipid from microalga Scenedesmus sp. with cellulase, xylanase and pectinase, using various parameters, such as enzyme concentration, temperature, pH and incubation time, was optimized by central composite design (CCD) coupled with response surface methodology (RSM). Both the lipid extraction from microalgae and the fatty acid methyl esters (FAMEs) production under optimal conditions showed a ∼twofold in the yields compared to the control group with no enzymatic treatment. SEM images, FTIR measurement, XPS and HPLC analysis showed that the enzymatic pretreatment caused significant alterations in the cell wall structure of microalgae. And the disruption of microalgal cell walls was primarily attributed to the breakage of β-glucosidic linkages in cellulose and hemicellulose. The study showed a promising approach can lead to an improvement in the lipid extraction yield from microalgae and further provide valuable information for the use of enzymes in microalgal processes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
