Abstract
Fungal isolate Aspergilius niger V-8 (IITB) was isolated from the sludge of anaerobic digester of distillery wastewater treatmentplant and was found to be an appropriate fungal isolate for the purposes of decolourization of anaerobically digested molassesspentwash (ADMS). The study aimed at optimization of the environmental parameters for achieving maximum decolourizationof ADMS using isolated fungal species. Environmental parameters like initial pH, mixing, immobilization and surfactant additionwere studied and their optimal conditions were assessed. Results show that initial pH 5.5, well mixed suspended growth withoutaddition of any surfactant were best suited for growth of isolate Aspergilius niger V-8 (IITB).
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