Abstract
The goal of this study was to find suitable composition of cultivation media for enniatin production by isolated endophytic strain Fusarium dimerum. In order to find optimal cultivation media, mono- and di- saccharides, complex nitrogen sources and L-amino acids directed biosynthesis of enniatins were tested. Submerged cultivation experiments were carried out in cultivation flasks. Most promising medium for enniatin accumulation contained fructose, malt extract and peptone for bacteriology. Finally, quite expensive carbon source fructose was replaced by more available syrups. Optimization resulted in 4-times elevated enniatin biosynthesis by metabolites production microorganism. Moreover, this is the strain obtained from Magnolia soulangeana, which has similar metabolites spectrum as the isolated Fusarium dimerum. Comparison of these results with published ones revealed that this endophyte is a potential strain for enniatins biosynthesis in submerged cultivation in which the maximum accumulation 1.27 g.L-1 of enniatin in culture medium was reached in a short period (96 h). The results proved that the endophytic strain F. dimerum may potentially be applied for efficient production of bioactive enniatins.
Highlights
Enniatins are cyclic hexadepsipeptides composed of N-methyl L-amino acids and
D-2-hydroxyisovaleric acid produced by microorganisms classified in Fusarium, Alternaria, Verticillium and Halosarpheia genera
F. dimerum was grown for two days on a rotary shaker (3.7 Hz; 180 rpm) at 28 °C in the dark and a 10 mL portion of vegetative culture was inoculated into the flat bottom cultivation flasks (500 mL) with working volume 100 mL of liquid broth
Summary
D-2-hydroxyisovaleric acid produced by microorganisms classified in Fusarium, Alternaria, Verticillium and Halosarpheia genera. These compounds are known for their ionophoric effect which is involved inter alia in their antibiotic, antifungal, phytotoxic and anthelmintic activity. They have been investigated for their potentials as anti-HIV and anti-cancer agents, the latter due to their discovered new mechanisms of pro-apoptotic activity (McKee et al., 1997; Dornetshuber et al, 2007; Meca et al 2010). Pdr5p a functional homologue of mammalian P-glycoprotein, one of the major efflux pumps involved in multidrug resistance (Hiraga et al, 2005; Yamamoto et al, 2005). They have chemically stable properties, serve as indicators of fungal contamination of food grains and are required as an analytical standards worldwide (Sorensen et al, 2008; Malachova et al, 2011; Sifou et al, 2011)
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