Optimization of elicitation condition with Jasmonic Acid, characterization and antimicrobial activity of Psoralen from direct regenerated plants of Psoralea corylifolia L

Abstract

Abstract In present study, estimation and characterization of Psoralen were done for Jasmonic Acid treated in vitro plants of Psoralea corylifolia L. The elicitor Jasmonic Acid at different concentrations (50,100,150, 200 and 250 µM/L) was used along with 6% sucrose and plant growth regulators to enhance psoralen content in leaves and roots of P. corylifolia . Shoot tip explant cultured on MSB 5 medium containing 2.0 mg/L BAP showed higher induction and proliferation of multiple shoots with 82.2% of response and 16.3 shoots/explant. In vitro rooting was best achieved with 82.6% of response and 18.6 roots/shoot at 0.8 mg/L IAA. JA at 150 µM/L concentration was found to enhance Psoralen content in leaves and roots of in vitro regenerated plants of P. corylifolia . The presence and structure of psoralen in methanolic extract of leaves and roots were confirmed through HPLC (retention time 21.007 in root, 20.659 in leaf sample), UV (213 nm), FT-IR, and NMR (C 11 H 6 O 3 ). The amount of Psoralen in JA treated leaf and root sample were found to be higher 1.17 mg/mL and 3.93 mg/mL respectively when compared to naturally growing P. corylifolia (0.56 mg/mL). The methanolic extract of root sample showed effective antimicrobial activity against tested bacterial and fungal pathogens. The present work provides the possibility of increasing production of psoralen in in vitro plants of P. corylifolia using Jasmonic Acid as an elicitor.