Optimization of Dyes Production by Mixed Aspergillus and Paecilomyces and Its Coloring on Cotton Cloth

Abstract

The present study aimed to optimize dyes of mixed Aspergillus and Paecilomyces and to evaluate dyes of the fungi in coloring cotton cloth. Different initial pH values (3, 5, 7, and 9), temperatures (24, 27, and 30°C), carbon sources (lactose, glucose, and sucrose,) and nitrogen sources (monosodium glutamate, potassium nitrate, sodium nitrate, and yeast extract) of the medium related to dyes and biomass production; and they coloring on cotton cloth were analysed. The optimum culture conditions for the dyes production by the mixed fungi was achieved at pH 9 (4.074 ± 0.0621 UA/L), a temperature of 24°C (4.4145 ± 0.1530 UA/L), with sucrose (4.1503 ± 0.0711 UA/L) as a carbon source, and sodium nitrate (4.0730 ± 0.0459 UA/L) as a nitrogen source, while for the maximum biomass production was obtained at pH 5 (3.7303 ± 0.1432 g/L), a temperature of 30°C (4.2997 ± 0.0372 g/L), with sucrose (2.965 ± 0.5431 g/L) as a carbon source, and monosodium glutamate (4.2697 ± 0.2843 g/L) as a nitrogen source. Culture conditions generated various shades on cotton cloth dyed with the fungal dyes. The intensity of color produced on the dyed cotton cloth by the fungal dyes was in line with the concentration of the dyes.