Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best extraction method, sample conservation and starting tissue

Abstract

Methodological studies are an important previous step in molecular research. We optimize a method for DNA extraction from eggs and a noninvasive extraction method for adult tissues of Maja brachydactyla. Our results show that lyophilized eggs in B developmental stage are the most suitable for DNA extraction. Regarding adult tissues, we propose setae as an alternative tissue for DNA extraction, since prevents mutilation of specimens, which avoids infections and fitness reduction. That is especially relevant in studies with endangered species of crustaceans.