Optimization of Degrading Poly-Containing Wastewater Conditions of Mixed Bacteria by Orthogonal Experiments

Abstract

The factor influencing on the mixed bacteria to degrade wastewater containing poly-containing conditions were optimized. Crude oil as a source of carbon, the effects of nitrogen sources, phosphorus sources, time, inoculation amount, temperature, mineralization content, initial pH value and activation time on degrading poly-containing wastewater were investigated by experiment of single factor and orthogonal experiments. The optimum process conditions were as follows: polyacrylamide (HPAM) which comes from waste water as a source of nitrogen, without other nitrogen source, K2HPO4 was phosphorus sources, the microorganisms activation time was 2, incubation time was 3d, initial pH value was 7, the inoculum size was 5%(v/v), incubation temperature was 35°C, the mineralization degree was 4000 mg•L-1. The main factors affecting degradation rate was nitrogen sources, time, pH, activation time, inoculum size and mineralization content.