Abstract
Background: Current esophageal treatment is associated with significant morbidity. The gold standard therapeutic strategies are stomach interposition or autografts derived from the jejunum and colon. However, severe adverse reactions, such as esophageal leakage, stenosis and infection, accompany the above treatments, which, most times, are life threating. The aim of this study was the optimization of a decellularization protocol in order to develop a proper esophageal tissue engineered construct. Methods: Rat esophagi were obtained from animals and were decellularized. The decellularization process involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) buffers for 6 h each, followed by incubation in a serum medium. The whole process involved two decellularization cycles. Then, a histological analysis was performed. In addition, the amounts of collagen, sulphated glycosaminoglycans and DNA content were quantified. Results: The histological analysis revealed that only the first decellularization cycle was enough to produce a cellular and nuclei free esophageal scaffold with a proper extracellular matrix orientation. These results were further confirmed by biochemical quantification. Conclusions: Based on the above results, the current decellularization protocol can be applied successfully in order to produce an esophageal tissue engineered construct.
Highlights
Esophageal disease-related morbidity has increased dramatically in the last 10 years
Histological Analysis rat esophagi (rES) were successfully decellularized with the current protocol
After the first decellularization cycle, rES were characterized by well-preserved extracellular matrix (ECM), while cellular and nuclear materials were eliminated (Figure 1)
Summary
Esophageal disease-related morbidity has increased dramatically in the last 10 years. Autologous grafts from the stomach, jejunum or colon can be applied, but 40% of patients die due to serious adverse reactions, such as limited nutrition and esophagus infection [6]. The aim of this study was the optimization of a decellularization protocol in order to develop a proper esophageal tissue engineered construct. Results: The histological analysis revealed that only the first decellularization cycle was enough to produce a cellular and nuclei free esophageal scaffold with a proper extracellular matrix orientation. These results were further confirmed by biochemical quantification. Conclusions: Based on the above results, the current decellularization protocol can be applied successfully in order to produce an esophageal tissue engineered construct
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