Abstract

Effect of culture conditions (temperature, initial pH value and volume) on the bioaccumulation of selenium (Se) in yeast ( Saccharomyces cerevisiae) were investigated by response surface methodology (RSM) in this paper. The combined effects of culture conditions on Se yield were studied using a three-level three-factor Box–Behnken design. Fermentation was carried out at different temperature (24–32 °C), initial pH value (4–7) and volume (40–100 mL). The results showed that the optimum conditions for Se enrichment of yeast were found at temperature 27.4 °C, initial pH value 5.8 and volume 89.4 mL. Total Se yield was significantly affected by culture temperature ( P < 0.05), initial pH value ( P < 0.01) and volume ( P < 0.01). Using a culture medium supplemented with 15 μg/mL sodium selenite (Na 2SeO 3) added at 9 h after inoculation which is the logarithmic growth phase, the maximum biomass and total Se yield in yeast could reach 9.23 g/L and 5.90 mg/L, respectively, which were significantly higher ( P < 0.05) than that of the control (8.82 g/L and 4.31 mg/L)

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