Optimization of culture medium compositions for gellan gum production by a halobacterium Sphingomonas paucimobilis

Abstract

The effect of culture medium compositions on gellan gum production produced by fermentation with a halobacterium Sphingomonas paucimobilis QHZJUJW CGMCC2428 was studied. In this work, a fractional factorial design was applied to investigate the main factors that affected gellan gum production by S. paucimobilis QHZJUJW CGMCC2428. Sucrose was the best carbon source for gellan gum and peptone displayed better inducing effect. Central composite design and response surface methodology were adopted to derive a statistical model for optimizing submerged culture medium composition. These experimental results showed that the optimum culture medium for producing gellan gum was composed of 40.00 (w/v) sucrose, 3.00% peptone (w/v), MgSO4 (w/v), 9.20% KH2PO4 (w/v), 7.50% Na2HPO4 (w/v), 4.30% K2SO4 (w/v), pH 6.8–7.0. The maximal gellan gum was 19.89±0.68g/L, which was agreed closely with the predicated value (20.12g/L). After incubated for 72h under the optimized culture medium in 5-L bioreactor, the gellan gum fermentation reached about 19.90±0.68g/L, which was higher than that in the initial cultivation medium.