Optimization of Culture Conditions ,Separation and Purification of a Lipopeptide Biosurfactant from <i>Pseudomonas palleronii S6X</i>

Abstract

The influences of environmental factors on biomass and yield of biosurfactant were investigated by the single factor experiment. The optimal culture conditions ofPseudomonas palleroniiS6Xare: sucrose as carbon source, soy peptone as nitrogen source, 0.5% NaCl, pH7.0. The optimal fermention conditions of Pseudomonas palleronii S6X strain are: 3.0% inoculum, 30°C, 50ml medium volume(250ml flask). Under this condition, maximal biomass increase 17.1%, biosurfactant yield increase 33.3% Crude product biosurfactant was obtained by acid precipitation method, and the output is 1.33g/l. Crude product biosurfactant was extracted with dichloromethane and lyophilized. The lyophilized product of biosurfactant was isolated by Sephadex LH-20 column, the yield of primary purification product was reached 82.5%The primary purification product was purified by ODS column further, the yield of second pure product was reached 70%The pure product was identified as a lipopeptide by TLC.