Abstract
The present study aimed to optimize culture conditions for optimal growth and production of extracellular lipase. Lipolytic fungal strain named as S3St2 previously isolated from a petrol pump soil sample of Newai Town was used for optimization study. Among the tested carbohydrate carbon sources, polysaccharide-starch exhibited maximum lipase production (21.25±0.70 IU/ml/min) with highest specific activity (1.47±0.06 U/mg). Lipase activity and specific activity were higher with mustard oil 1 % (v/v) among all lipidic carbon sources. Among inorganic nitrogen source, potassium nitrate was found best inducer of lipase activity, malt extract supported the fungus growth (dry weight of cell pellets was 0.467 g) and exhibited maximum lipase activity among all organic nitrogen sources. Lipase activity was optimum at pH 8.0, indicates alkalophillic nature of production media supports the growth of fungus. Higher lipase activity (27.92±0.87 IU/ml/min) was detected at 28ºC. The incubation time of 5 days was found optimum for maximum lipase production (31.51±0.21 IU/ml/min).
Highlights
Lipases (EC 3.1.1.3) are triacylglycerolester hydrolases that cleave the triaclyglycerol into glycerol and fatty acids
The lipolytic fungal strain named as S3St2 (Fig. 1) was obtained from the Department of Bioscience and Biotechnology, Banasthali Vidyapith which was previously isolated from the petrol pump soil sample of Newai Town (Raj) and screened for lipolytic activity on tributyrin agar (TBA) plates
Starch is a kind of polysaccharide supported lipase production by fungus, used in further optimization studies
Summary
Lipases (EC 3.1.1.3) are triacylglycerolester hydrolases that cleave the triaclyglycerol into glycerol and fatty acids. They frequently exhibit other properties such as amidase, phospholipase, cutinase, cholesterol esterase and isophospholipase [1]. Lipases are mainly found in fungi, yeasts, bacteria, plants and animals [2,3,4]. At present microbial lipases plays a vital role and potential applications in industry, mostly in the dairy, detergents, oils, fats and pharmaceutical industries due to their potential towards extremes of pH, organic solvents, temperature, chemoselectivity and regioselectivity [5,6]. Qualitative and quantitative improvement demands enhanced due to increase in application of lipases in different fields. Lipases posses specificity to a reaction and makes postreaction simpler
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