Abstract
In this study, optimization of growth, lipid and DHA production of Aurantiochytrium SW1 was carried out using response surface methodology (RSM) in optimizing initial fructose concentration, agitation speed and monosodium glutamate (MSG) concentration. Central composite design was applied as the experimental design and analysis of variance (ANOVA) was used to analyze the data. ANOVA analysis revealed that the process which adequately represented by quadratic model was significant (p < 0.0001) for all the response. All the three factors were significant (p < 0.005) in influencing the biomass and lipid data while only two factors (agitation speed and MSG) gave significant effect on DHA production (p < 0.005). The estimated optimal conditions for enhanced growth, lipid and DHA production were 70 g/L fructose, 250 rpm agitation speed and 10 g/L MSG. Consequently, the quadratic model was validated by applying the estimated optimum conditions, which confirmed the model validity where 19.0 g/L biomass, 9.13 g/L lipid and 4.75 g/L of DHA were produced. The growth, lipid and DHA were 28, 36 and 35% respectively higher than that produced in the original medium prior to optimization.
Highlights
Fructose was used instead of glucose and other carbon sources is in conjunction from our previous experiment which concluded that fructose is the best carbon sources for growth, lipid and DHA production by this Thraustochytrids strain[17]
The profiles of biomass, lipid, DHA, as well as fructose assimilation of Aurantiochytrium SW1 cultured in 500 mL flask containing 100 mL of medium, was demonstrated in Fig. 1a and b
This figure showed that, the influence of each factor for all the response varied among each other and it was concluded that agitation speed had the most significant impact on biomass productions, whereby fructose and monosodium glutamate (MSG) had pronounced impact for lipid and DHA production respectively
Summary
Yusuf Nazir[1,3], Shuwahida Shuib[1], Mohd Sahaid Kalil[2], Yuanda Song3 & Aidil Abdul Hamid[1]. The estimated optimal conditions for enhanced growth, lipid and DHA production were 70 g/L fructose, 250 rpm agitation speed and 10 g/L MSG. Optimization of culture conditions has been proven to significantly improved growth and DHA production by most Thraustochytrids including Aurantiochytrium sp[14,15]. This study intends to optimize the culture condition to enhance the growth, lipid and DHA production of our Thraustochytrids isolate. Optimizing the carbon, nitrogen and agitation speed are key factors to achieve high cell growth as well as enhanced lipid and DHA production by Auratiochytrium sp. The aim of this study is to optimize fructose and MSG which are the major carbon and nitrogen source in the cultivation media as well as agitation speed to enhance growth, lipid and DHA production of locally isolated Auranthiochytrium SW1 by using RSM. Fructose was used instead of glucose and other carbon sources is in conjunction from our previous experiment which concluded that fructose is the best carbon sources for growth, lipid and DHA production by this Thraustochytrids strain[17]
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