Optimization of cultural conditions for submerged state fermentation of di-gested biogas slurry for production of lignocellulolytic enzymes using Phanaerochaete chrysosporium MTCC 787

Abstract

Growing environmental concerns and increasing demands from end-use sectors have increased the glob-al market for microbial products. Optimizations of production parameters hold great importance for the industry. The present study was aimed at optimization of submerged state fermentation conditions for production of lignocelluloly-tic enzymes from digested biogas slurry by Phanaerochaete chrysosporium MTCC 787. Enzyme activities for differ-ent enzymes i.e. endoglucanase, exoglucanase, ?-glucosidase; xylanase and mannanase; laccase, lignin peroxidase and manganese peroxidise, using P. chrysosporium MTCC 787 were maximum at 50% concentration of digested slur-ry and showed maximum value of xylanase i.e. 187.41U/ml. Effect of temperature (25°C, 30°C and 35°C) on lignocellu-losic bioconversion showed that at 30°C, maximum value of manganese peroxidise (167.5 U/ml) was obtained. High-est enzyme activites were obtained at selected inoculum size i.e. 10?spores/ml, e.g. 85.29 U/ml xylanase was ob-tained. Incubation period of 8 days and pH of 7.0 came out to be best conditions for P. chrysosporium MTCC787 to produce maximum enzyme activity e.g. xylanase 95.47U/ml at pH 7.0 and xylanase 144.96U/ml at 8 day incu-bation.This work presents a novel concept in optimization of fermentation process to produce lignocellulolytic en-zymes as this work is focussed on utilization of digested biogas slurry as a substrate for enzyme production and enhancement of the production with microbial source, which is environment friendly.