Abstract

Abstract Production of phycocyanin from Spirulina platensis by mixotrophic condition was favored due to high growth rate result. The objective of this research was to obtain the statistical interaction models of molasses and urea to crude phycocyanin (CPC) production by applying response surface methology (RSM)associated central composite design approach. S. platensis was cultivated under mixotrophic condition by adding varied molasses and urea under continous illumination at 45.5 μmol m−2 s−1 for 6 days. By using 2nd order statistical models, optimum phycocyanin production was recorded on 114.74 mg L−1 and 0.196 L−1 of urea and molasses, respectively. Molasses could be the promising substrate for phycocyanin production of the microalgae.

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