Optimization of core phases of biolistic transformation of potato

Natalya P Malakhova,Elina R Maltseva,Yuriy A Skiba,Gulnara A Ismagulova,Botakoz K Tezekbayeva,D Solovyev,G Burygin

doi:10.1051/bioconf/20202301007

Natalya P Malakhova, Elina R Maltseva + Show 5 more

Open Access

https://doi.org/10.1051/bioconf/20202301007

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Abstract

We have established the optimal exposure time for internodes and callus plant material of Aksor potato variety on the OSS osmotic medium before and after biolistics and the type of plant explant that is most suitable for that. The optimal objects were explants from internodes of test plants. The best time for exposure of plant material to the osmotic medium before bombardment is 24 hours, and after biolistics – 48 hours. Callus tissue developed on the MS medium with zeatin two weeks later in internodes that were cultivated before bombarding on the OSS for 24 hours and we observed an increase in the callus mass by the end of fourth week. Not a single callus was obtained with rhizogenesis on the R4 medium from internodes and potato calli that were on the osmotic medium within 24 and 72 hours after biolistics. The regenerative capacity of internodes after cultivation on an osmotic medium for 48 hours after bombarding was higher than that of calli. We obtained 12 regenerant plants out of 200 internode explants and 4 regenerant plants out of 150 calli that were used in the experiment.

Highlights

The gene pool of potatoes (Solanum tuberosum) in the Republic of Kazakhstan consists of 1,450 samples from the worldwide collection from 32 countries and includes samples of wild and cultivated species, interspecific hybrids, as well as domestic and foreign varieties [1]
Most experiments on biolistic transformation of potatoes are aimed at creating new lines with increased yield and resistance to fungal and bacterial pathogens
An analysis of the results showed that the optimal time for explants to be located on the OSS medium after biolistics is 48 hours

Summary

Introduction

The gene pool of potatoes (Solanum tuberosum) in the Republic of Kazakhstan consists of 1,450 samples from the worldwide collection from 32 countries and includes samples of wild and cultivated species, interspecific hybrids, as well as domestic and foreign varieties [1]. Due to its exceptional qualities, such as high yields, high content of carbohydrates, protein and vitamins, the demand for potatoes is constantly increasing compared to the other crops [2]. As one of the leading crops in agriculture, potatoes serve as an important object for scientific research conducted in various areas of modern biotechnology. One of the pressing issues associated with the successful cultivation of potatoes, along with proper agricultural technology, is the use of varieties that are resistant to the action of various stress factors of abiotic and biotic nature. Most experiments on biolistic transformation of potatoes are aimed at creating new lines with increased yield and resistance to fungal and bacterial pathogens. Plants with an average increased yield of 50-80% were obtained [3,4,5]

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