Abstract
In this study, factors affecting the extraction yield of carotenoids from dry shrimp waste by organic solvents such as ratio of hexane / acetone, ratio of solvent / shrimp waste, extraction temperature, extraction time, extraction method such as dynamic or static have been studied. The results showed that the solvent ratio hexane: acetone = 3: 1 gave the highest carotenoid yield. In this ratio of solvent’s mixture, the yield reached highest at temperature 60 °C for 2 hours, which was 44,64 µg / g raw shrimp waste (d.b.) (ratio of solvent to raw material 3/1). Ultrasound or vortexing gave higher extraction yield than in static conditions, which was 1.5- to 1.8- fold increase, respectively. At the ratio of solvent: dried shrimp = 4: 1, the amount of carotenoid recovered at 60°C for 2 hours reached 57.4 µg / g. However, if the shrimp waste was hydrolyzed with Alcalase at 50°C for 4 hours before extraction by solvent, the amount of carotenoid recovered achieved 149 µg / g of raw material
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