Abstract
Application of crude xylanolytic and pectinolytic enzymes in diverse industrial processes make these enzymes commercially valuable and demand their production process to be cost-effective. Out of four different agrowaste biomass, wheat bran (WB) and citrus peel (CP), when amended as fermentation substrates, respectively induced the highest xylanolytic enzymes and pectinolytic enzymes from both, B. safensis M35 and B. altitudinis J208. Further, the simultaneous amendment of WB and CP yielded concurrent production of these cellulase free xylanolytic and pectinolytic enzymes. Hence, the quadratic model was developed using the Central Composite Design of Response Surface Method (CCD-RSM). The model gave the concentration values for WB and CP substrates to be amended in one single production medium for obtaining two optimized predicted response values of xylanase activity and pectinase activity units, which were further practically validated for the xylanase and pectinase production responses from the optimized production medium (OPM). These practically obtained response values from OPM were found to be in accordance with a range of 95% predicted intervals (PI) values. These observations verified the validity of the predicted quadratic model from RSM and suggested that both xylanase and pectinase enzymes can be induced concurrently from both of the bacterial strains.
Highlights
Xylanases and pectinases are the commercially important industrial groups of enzymes, members of which exhibit diverse xylanolytic and pectinolytic enzymatic activities
In comparison with basal Bushnell Haas Medium (BHM)-YEP medium alone or basal BHM-YEP amended with glucose (G), supplementation of each polysaccharide substrate to basal BHM-YEP individually resulted in increased growth for both the isolates
The growth further increased in this order, citrus peel (CP), rice bran (RB), cotton seed cake (CSC), molasses (M), wheat bran (WB), pectin (Pn) and resulted in highest on supplementation of xylan (Xn)
Summary
Xylanases and pectinases are the commercially important industrial groups of enzymes, members of which exhibit diverse xylanolytic and pectinolytic enzymatic activities. Pulp and paper as well as textile industries need the cellulase free xylanase and pectinase enzymes for their respective applications, i.e., to prepare hemicellulose free cellulose papers, to remove pectin (rhamnogalacturonan) coating from cotton and denim fibers, Food and beverage industries need xylanase and pectinase enzymes to clarify pectin polymeric fraction from fruit juices and to improve the tea flavor[1,2]. These applications append the worth to the organisms which can produce the xylanase and pectinase enzymes and the cellulase free nature of such enzymes is an add-on benefit to this
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
