Abstract
Graphical methods are described for determining suitable experimental conditions for optimum chromatographic resolution of complex mixtures. Plotting the logarithm of retention time for individual components or retention time ratios for close eluite pairs as a function of an experimental variable such as solvent strength or temperature allows direct visual evaluation of the best resolution attainable with a given column of known efficiency. Optimization of temperature and solvent strength for the separation of phenylthiohydantoin amino acids is demonstrated for two reversed-phase columns.
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