Optimization of Colloidal Chitin and Inoculum Concentration in Chitinase Production byStreptomycessp. PB2 Using Response Surface Methodology

Eri Pramesti,Indun Dewi Puspita,A Isnansetyo,A Dwi Jayanti,R.C Bhujel,N Huda,Mgs M Prima Putra,R Ida Adharini,D Wahyu Kartika Sari,I Dewi Puspita

doi:10.1051/e3sconf/202014703011

Abstract

Chitinase is an enzyme capable of catalyzing the hydrolysis of chitin. Bacteria is one of the sources for chitinase and the modification process of production are continously developed.Streptomycessp. is one of Actinomycetes group that shows high activity in hydrolyzing chitin. This study aimed to find the optimal conditions ofStreptomycessp. PB2 for producing chitinase at various colloidal chitin (0.5%, 1%, 1.5%) and inoculum (0.5%, 1%, 1.5%) concentration in the chitin broth medium using Respons Surface Methodology (RSM). The examined parameters included Total Plate Count (CFU/ml) and chitinase activity (U/ml). Chitinase activity was statistically analyzed by MiniTab 17 to obtain a mathematical model, then were validated. The mathematical model of chitinase activity was Y = -0.000075 + 0.00056 K + 0.00067 I, with the optimum colloidal chitin concentration (K) of 1.5% and inoculum concentration (I) of 1.5%. The highest chitinase activity of 0.0019 U/ml in day-2 fermentation. The validation test showed that the mathematical model had a low accuracy with the SSE value of 0.5306. This study shows that colloidal chitin and inoculum concentration are important factors to be optimized. However, further model is needed to be developed for a better estimation of process.

Highlights

Chitinase is an enzyme catalyzing the hydrolysis of ß-1,4-glycosidic bond of chitin
For the inoculum concentration of 103 – 106 CFU/ml were used for the production of chitinase by Streptomyces sp
This study shows that optimization of colloidal chitin and inoculum concentration in the medium accelerated the period of chitinase production by Streptomyces sp

Summary

Introduction

Chitinase is an enzyme catalyzing the hydrolysis of ß-1,4-glycosidic bond of chitin. It is well-known enzyme having capability to produce value-added product derived from shrimp shell waste such as chitin and/or chitosan oligomers, N-acetylglucosamine, and glucosamine which can be applied in food, cosmetics, and biomedical industries [1] [2]. Hydrolysis of chitin is carried out through the enzymatic or chemical process. Enzymatic hydrolysis, in which chitinolytic microorganism plays an important role in this process, is preferable compare to the chemical process as it offers an eco- friendly process, more affordable, and easier to be handled [3]. 90-99% chitinolytic bacteria population belongs to the group of Actinomycetes [4].

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