Abstract
In order to increase the water solubility of chitosan and potential application to products that is good for human health. Chitosan was caried out the optimization of hydrolysis by cellulase to produce chitooligosaccharide. Chitosan with degree of deacetyl more than 80% and cellulase used in this study. Surface Response Method (RSM) - Central Composite Design (CCD) option is used to optimize the hydrolysis. Results of the study showed optimal values for hydrolysis such as 49 oC temperature, 5.9 pH, 0.76% substrate concentration, and 8.97 U/g enzyme concentration, 180 minutes hydrolysis time. More than 90% of the oligosaccharides produced were in the range less than 10 kDa. The research results are the premise for production of COS powder to water-soluble.
Highlights
potential application to products that is good for human health
Chitosan was caried out the optimization of hydrolysis
more than 80% and cellulase used in this study
Summary
Chitosan (mức độ deacetyl > 80%) được cung cấp bởi công ty Chitosan Việt Nam, Số 23/6 Ngô Thời Nhiệm, Rạch Giá, Kiên Giang. Acid lactic 88% dùng trong thực phẩm (India), NaHCO3 (India), Acid-3,5-dinitrosalicylic [DNS] (Merck)
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