Abstract
Polysaccharides from Taraxacum mongolicum were extracted using cellulase-assisted extraction technology. Based on the results of single factor experiments, three independent variables extraction time, temperature and pH were selected for investigation. Response surface methodology (RSM) was used to optimize the extraction conditions. The optimal conditions were as follows: extraction time, 105.07 min; extraction temperature, 47.49°C; extraction pH, 4.81. Under such conditions, the yield of polysaccharides was 13.75% (w/w), and the yield obtained by cellulase-assisted extraction method was remarkably higher than the hot water extraction. In vitro assays, scavenging activity of 1, 1-Diphenyl-2-picrylhydrazxyl (DPPH) radical and superoxide anion radical showed that the polysaccharides from T. mongolicum had definite antioxidant activities, and polysaccharides extracted from different parts of T. mongolicum had different antioxidant activity, which shows: inflorescence > leaf > root. These results exhibit that cellulase-assisted extraction is an advisable method for T. mongolicum polysaccharides and polysaccharides could be explored as potential antioxidant to use in medicine or functional food.
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