Abstract

Water hyacinth was used as substrate for bioethanol production in the present study. Combination of acid pretreatment and enzymatic hydrolysis was the most effective process for sugar production that resulted in the production of 402.93 mg reducing sugar at optimal condition. A regression model was built to optimize the fermentation factors according to response surface method in saccharification and fermentation (SSF) process. The optimized condition for ethanol production by SSF process was fermented at 38.87°C in 81.87 h when inoculated with 6.11 ml yeast, where 1.291 g/L bioethanol was produced. Meanwhile, 1.289 g/L ethanol was produced during experimentation, which showed reliability of presented regression model in this research. The optimization method discussed in the present study leading to relatively high bioethanol production could provide a promising way for Alien Invasive Species with high cellulose content.

Highlights

  • The major source of energy comes from non-renewable fossil fuel that caused global warming, environmental degradation, and human health problems (Patil et al, 2014)

  • Hemicelluloses and lignin contents in water hyacinth were determined by the methods of Goering and Van soest (1970)

  • The average value of moisture content in water hyacinth sample was 90.85%, which is much higher than the other cellulosic wastes

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Summary

Introduction

The major source of energy comes from non-renewable fossil fuel that caused global warming, environmental degradation, and human health problems (Patil et al, 2014). Water hyacinth (Eichornia crassipes), which is originated from Amazon basin (Barrett, 1989), is listed as one of the world’s most invasive and recalcitrant weeds because of its availability in large quantities, extraordinary adaptive ability, and remarkable growth rate (Hu et al, 2015). It grows at an extreme rapid rate and produce almost 2 tons of biomass per acre and its population doubles every 5–15 days (Craft et al, 2003). Water hyacinth is usually blamed for depleting nutrients and oxygen from water bodies, increasing evapotranspiration, and reducing biodiversity, which could

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