Abstract
Lignocellulosic materials are the largest component of biomass in the world, and therefore suitable substrates for anaerobic digestion (AD). However, they are recalcitrant due to lignin-encrustation and the crystallinity of cellulose. Utilizing the wealth of knowledge in literature, this study compares different bioaugmentation strategies for the AD of Axonopus compressus, common name cowgrass, from single cultures to an engineered mix of three microbes with different functions. An optimization experiment is carried out on the biochemical methane potential with the best combination resulting in 20.7% more methane. The combination is then validated in a reactor operated semi-continuously, which increased production relative to the control after 18 days. The community dynamics of the bioaugmented reactor was tracked by Illumina MiSeq and compared against that of the control. The results prove that a properly engineered bioaugmentation is capable of increasing methane yield from a lignocellulosic substrate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
