Optimization of banana juice extraction using combination of enzymes.

Abstract

Banana juice extraction was optimized using central composite rotatable design with four numerical factors was employed to design the experiments. The numerical factors were incubation temperature (30-50°C), and incubation time (20-60min), cellulase concentration (0-0.4g/100g banana) and pectinase concentration (0-0.4g/100g banana). The optimum condition for extraction of banana juice were incubation temperature of 36.5°C, incubation time of 29.33min, cellulase concentration of 0.34% and pectinase concentration of 0.35%. The corresponding values of yield, viscosity, lightness, acidity, reducing sugar content and total soluble solids at the optimum condition were 74.15%, 101.14mPas, 30.06, 2.72%, 171.28mg/100g juice and 12.10°Brix, respectively. Response surface analysis showed that yield increased with incubation time, and cellulase and pectinase concentration, and with temperature it initially increased and then decreased. The processing parameters had an opposite effect on viscosity. Reducing sugar content was also affected by all processing parameters.