Abstract
BackgroundAquaculture is one amongst the growing and major food producing sectors. Shrimp culture is one of the subsectors of aquaculture that attracts more attention because of the economic interest. However, the shrimp culture systems have been facing severe consequences and economical losses due to disease outbreaks. Risk of disease outbreak can be combated with the application of probiotics. For economically viable production of such probiotic products, the present study provides information on the optimization and partial purification of bacteriocin produced by a goat milk isolate Lactobacillus sp. MSU3IR against the shrimp bacterial pathogens.ResultsBacteriocin production was estimated as a measure of bactericidal activity (arbitrary Unit/ml) over the test strains. The optimum culture conditions and media components for maximum bacteriocin production by Lactobacillus sp. MSU3IR were: pH: 5.0, temperature: 30°C, carbon source: lactose; nitrogen source: ammonium acetate; NaCl: 3.0% and surfactant: Tween 80. MRS medium was found to extend better bacteriocin production than other tested media. Upon partial purification of bacteriocin, the SDS-PAGE analysis had manifested the presence of two peptide bands with the molecular weight of 39.26 and 6.38 kDa, respectively.ConclusionThe present results provide baseline trend for the statistical optimization, scale up process and efficient production of bacteriocin by the candidate bacterial strain Lactobacillus sp. MSU3IR which could be used to replace the usage of conventional chemotherapeutics in shrimp culture systems.
Highlights
Aquaculture is one amongst the growing and major food producing sectors
Infections caused by pathogenic strains belonging to the species Aeromonas hydrophila, Vibrio harveyi,V. parahaemolyticus, V.cholerae and V. anguillarium cause traumatic losses in the culture of molluscs, fish and shrimp [2,6,7,8,9]
Evaluation of probiotic bacteria capable of producing bacteriocin is becoming an area of rigorous research in several sectors of human nutrition, in animal husbandry and in fish farming [33]
Summary
Aquaculture is one amongst the growing and major food producing sectors. Shrimp culture is one of the subsectors of aquaculture that attracts more attention because of the economic interest. The shrimp culture systems have been facing severe consequences and economical losses due to disease outbreaks. Aquaculture has become a popular food producing sub sector complement to agriculture. Diseases caused by bacteria and viruses are considered to be an important problem in the intensive rearing of molluscs, finfish, lobster and shrimp [1]. Infections caused by pathogenic strains belonging to the species Aeromonas hydrophila, Vibrio harveyi,V. parahaemolyticus, V.cholerae and V. anguillarium cause traumatic losses in the culture of molluscs, fish and shrimp [2,6,7,8,9].
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