Optimization of atomic fluorescence measurements with a microcomputer-based time-multiplex multiple-slit spectrometer

Abstract

An automated multielement flame atomic fluorescence (AF) spectrometer is described. The instrument employs a multiple exit slit monochromator and a single detector. Each element is excited to fluoresce with a single-element hollow cathode lamp (HCL) and a time-multiplex mode is used for pulsing the HCLs and gated data acquisition. A microcomputer controls the pulsing of HCLs, sample introduction into the flame and data acquisition. Optimization of the HCL pulse width and peak current is shown to be critical. With an air/H 2 flame sheathed with Ar, the following single-element detection limits (in μg/ml) were obtained: Au, 0.2; Cd, 0.005; Co, 0.03; Cu, 0.003; Fe, 0.05; Mg, 0.0008; Mn, 0.007; Ni, 0.04; Pb, 1 and Zn, 0.02. Some multielement detection limits were a factor of 2–5 worse. At higher analyte concentrations, the signal to noise ratio decreased due to an increase in the relative analyte fluorescence flicker noise.