Abstract

Papaya has three types of efflorescense which are male flowers, female flowers, hermaphrodite. That was difficult to determine in advance for the type of flower that appear. The molecular technology approach is an effort to quickly predict the types of flowers that will appear. SNAP primers were designed based on SNP on genomic sequences of male papaya and hermaphrodite DNA. This research aimed to found the specific cycle and annealing temperature for PCR. The research was conducted at the in Indonesia Tropical Fruit Research Institute from January to March 2020. The SNAP primer used was T12, The plants used were papaya varieties of Pomegranate and local cultivar from male and hermaphrodite aged 8 months trees. The results showed that an annealing temperature of 59°C with a cycle of 28 times as effective in producing male DNA amplification on SNAP T2 reverse (R) primers and hermaphrodite DNA amplification plants appeared on alternate SNAP T12 primers (A) with a product size is 430 bp, while female plants did not occur. Amplification according to the SNAPT12 primer.

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