Abstract
Optimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL -1 were 30°C at pH 8.5 and incubation time 96 h in fr ee cell cultures using starch 20 g L -1 as carbon source and yeast extract 5 g L -1 as nitrogen source. The incubation time for the best yield of 344 unit mL -1 was reduced to 72 h under the optimized fermentati on conditions by immobilized cells adsorbed on synthetic cotton fibe rs. Data obtained during 5 reusable cycles showed h igher levels of enzyme in shorter time duration. Immobili zation of Streptomyces ambofaciens NRRL 2420 on synthetic cotton fiber permit repeated reuse of the cells under the optimized fermentation conditions.
Highlights
Protease enzyme catalyses the hydrolysis of proteins into peptides and amino acids and considered as one of the most useful enzyme groups
The incubation time for the best yield of 344 unit mL−1 was reduced to 72 h under the optimized fermentation conditions by immobilized cells adsorbed on synthetic cotton fibers
The effect of initial pH was significant on alkaline protease production (p
Summary
Protease enzyme catalyses the hydrolysis of proteins into peptides and amino acids and considered as one of the most useful enzyme groups. Alkaline proteases have found a wide application in several industrial processes, such as laundry detergents, protein recovery or solubilization as well as in the production of protein hydrolysates, meat tenderizations, in bating of hides and skins in leather industries, for the film industry and in waste processing (Gupta et al, 2002; Amit et al, 2011). It constitute 60-65% of the global industrial market (Banerjee et al, 1999). Streptomyces that produce protease include S. clavuligerus, S. griseus, S. rimosus, S. thermoviolaceus and S. thermovulgaris (James et al, 1991).
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