Optimization of a new selective pressurized liquid extraction methodology for determining organic pollutants in wild boar livers

Xiana González-Gómez,Noelia Cambeiro-Pérez,María Figueiredo-González,Elena Martínez-Carballo

doi:10.1016/j.mex.2021.101242

Xiana González-Gómez, Noelia Cambeiro-Pérez + Show 2 more

Open Access

https://doi.org/10.1016/j.mex.2021.101242

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Journal: MethodsX	Publication Date: Jan 1, 2021
Citations: 3	License type: cc-by

Affiliation: Universidade de Vigo

Abstract

In this study, a new selective pressurised liquid extraction (SPLE) methodology was optimised for determining about 70 organic pollutants (OPs) including organochlorine (OCPs), organophosphate (OPPs) and pyrethroid (PYRs) pesticides, polychlorinated biphenyls (PCBs), polybromodiphenyl ethers (PBDEs), as well as, polycyclic aromatic hydrocarbons (PAHs) in wild boar liver samples considering the temperature, pressure and time of contact between the solvent and the matrix as influential variables. Clean-up of extracts was performed by solid-phase extraction (SPE) using EZ-POP cartridges. Detection of OPs was carried out by gas chromatography (GC) coupled to tandem mass spectrometry (QqQ-MS/MS). This new approach offers:•A new non-time consuming SPLE methodology for determining about 70 OPs in wild boar.•Recoveries achieved ranged between 74 to 119 % with RSD less than 20 %.•Detection and quantification limits in the low to mid pg/g range.

Highlights

Environmental Science Biomonitoring environmental pollutants Selective pressurized liquid extraction X
The experimental runs were performed in 2.0 g liver samples spiked with organic pollutants (OPs) concentration range at 0.25 ng/g and 0.50 ng/g
Most of the target pollutants were detected in the selected liver samples with significant differences (p < 0.050) and the following mean level concentration order polycyclic aromatic hydrocarbons (PAHs) > OCPs > NDLPCBs > PYRs > OPPs > DLPCBs > polybromodiphenyl ethers (PBDEs)

Summary

Introduction

Environmental Science Biomonitoring environmental pollutants Selective pressurized liquid extraction X. The experimental runs were performed in 2.0 g liver samples spiked with OP concentration range at 0.25 ng/g and 0.50 ng/g. To extract the maximum target analytes with minimum interferences, different SPLE parameters were optimized.

Results

Conclusion